Characterization of Gossypol Degrading Enzymes from the Bacteria Isolated from the Gut of Pinkbollworm Larvae (Record no. 70804)

MARC details
000 -LEADER
fixed length control field 02597nam a2200217 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250207132755.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250207b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency UAS Dharwad
041 ## - LANGUAGE CODE
Language code English
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 630.24192
Author Label KAK
100 ## - MAIN ENTRY--PERSONAL NAME
Name of Author Kakol Kavita B.
245 ## - TITLE STATEMENT
Title Characterization of Gossypol Degrading Enzymes from the Bacteria Isolated from the Gut of Pinkbollworm Larvae
250 ## - EDITION STATEMENT
Edition Statement M.Sc. (Agri)
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of Publisher Dharwad
Name of Publisher University of Agricultural Sciences
Publication Year 2024
300 ## - PHYSICAL DESCRIPTION
Book Pages 69
Book Size 32 Cms
520 ## - SUMMARY, ETC.
Abstract. ABSTRACT<br/><br/> Cotton (Gossypium spp.) is a major agricultural crop grown in the world. Cottonseed meal (CSM) is the by-product of cotton obtained after seeds are crushed and which are rich in quality protein. However, its consumption is limited because of the presence of gossypol which is highly toxic to monogastric and ruminant animals which cause growth depression, abnormalities to intestine, reproductive organ and other internal organs. As they are rich source of protein and could be used as an alternate protein source for human and animal consumption, provided it is detoxified from gossypol and careful removal of fiber. Consumption of gossypol free glandless cotton seed flour promoted the growth, increased weight gain and a positive nitrogen balance. Therefore, the study was aimed to isolate gut bacteria from P. gossypiella and optimize the culture conditions and characterize the gossypol degrading enzyme and validate the biodegradation of gossypol in CSM by SSF. The culture conditions for the four gut bacterial isolates P1, P4, P12 and P13 were optimized and production of gossypol degrading enzyme was found maximum when 0.6g/L of gossypol was provided as sole source of carbon, inoculum level 2%, medium pH 8.0 and incubation temperature 30 oC. Optimum pH, temperature required for the gossypol degrading enzyme activity was pH at 9.0, 30 oC. Electrophoresis indicated that there were several protein bands obtained in the enzyme preparation with molecular weight from 29 to 124 kDa. The optimum fermentation conditions of CSM were found to be IMC 70%, pH 9.0, temperature 30oC and inoculum level 30%. The results indicated that the isolated bacteria were able to utilize gossypol as sole source of carbon in CSM and found to increase production of gossypol degrading enzyme and thus could be implicated in biodegradation of gossypol in CSM to enhance it’s utilization for consumption.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Subject Agricultural Biochemistry
700 ## - ADDED ENTRY--PERSONAL NAME
2nd Author, 3rd Author Akbar S. MD.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha Item type THESIS
Edition M.Sc. (Agri)
Classification part 630.24192
Call number prefix KAK
Suppress in OPAC No
942 ## - ADDED ENTRY ELEMENTS (KOHA)
-- 630_241920000000000
999 ## -
-- 70804
-- 70804
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Dewey Decimal Classification     University of Agricultural Sciences, Dharwad University of Agricultural Sciences, Dharwad 28/11/2024   630.24192/KAK T14035 07/02/2025 1 07/02/2025 THESIS