In planta Transformation of Ectabcgene Cluster For Salt Tolerance In Rice (Record no. 71078)

MARC details
000 -LEADER
fixed length control field 02632nam a22002057a 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250626122329.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250626b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency University of Agricultural Sciences, Dharwad
041 ## - LANGUAGE CODE
Language code English
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 630.2742
Author Label AJE
100 ## - MAIN ENTRY--PERSONAL NAME
Name of Author Ajeyans
245 ## - TITLE STATEMENT
Title In planta Transformation of Ectabcgene Cluster For Salt Tolerance In Rice
250 ## - EDITION STATEMENT
Edition Statement M Sc (Agri)
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of Publisher Dharwad
Name of Publisher University of Agricultural Sciences, Dharwad
Publication Year 2024
300 ## - PHYSICAL DESCRIPTION
Book Pages 80
Book Size 32 Cms
520 ## - SUMMARY, ETC.
Abstract. Salt stress significantly impacts global agricultural productivity, particularly in indica rice (Oryza sativa), which is known for its sensitivity to salinity. The popular rice variety BPT-5204, known for its excellent grain quality, matures in 140-150 days and has beenlocally preferred for decades. However, its performance declines significantly under saline conditions, with reduced yield. This study aimed to enhance salt tolerance in rice by introducing the ectABC gene cluster from Halomonas spp, responsible for ectoine biosynthesis, a key osmoprotectant.Osmoprotectant helps to mitigate salt stress bystabilizing cellular structures, maintaining osmotic balance, and protecting against oxidative damage.The ectABC cluster was subcloned into the plant expression vector pRI 101-AN and confirmed through restriction digestion and colony PCR. Using Agrobacterium tumefaciens strain LBA4404, the recombinant vector was successfully introduced into rice via in planta transformation by pricking the embryonic apical meristem with bacterial suspension using a sterileneedle.Transformationefficiency,basedonseedandseedlingregeneration,rangedfrom 1.25 per cent to 1.79 per cent respectively. PCR analysis confirmed transgeneintegration in five Tâ‚€ plants. Sequence homology analysis revealed 99.79 per cent identity withHalomonaselongata,affirmingthesuccessfulintegrationofthegenecluster.Thestudy outlines a comprehensive workflow for bypassing tissue culture, reducing associated complications such as somaclonal variation. The in planta approach demonstrated the feasibilityof direct T-DNA deliveryto the rice genome, simplifyingtransformation protocols. This research provides a foundation for engineering salt-tolerant rice, contributing to food security in saline-prone areas. By leveraging the ectABC gene cluster, this work highlights a sustainable solution for improving rice productivity under abiotic stress conditions, aligning with the goals of climate-resilient agriculture.
700 ## - ADDED ENTRY--PERSONAL NAME
2nd Author, 3rd Author Narayanmoger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha Item type THESIS
Edition M Sc (Agri)
Classification part 630.2742
Call number prefix AJE
Suppress in OPAC No
942 ## - ADDED ENTRY ELEMENTS (KOHA)
-- 630_274200000000000
999 ## -
-- 71078
-- 71078
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Dewey Decimal Classification     University of Agricultural Sciences, Dharwad University of Agricultural Sciences, Dharwad 20/03/2025   630.2742/AJE T14142 26/06/2025 1 26/06/2025 THESIS