000			02668nam a2200193 4500
003			OSt
005			20250106160927.0
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040			_cUAS Dharwad
041			_aEnglish
082			_a632.3 _bNIS
100			_aNishanth K.
245			_aIdentification and Characterization of Bacteria Endophytes Against Fusarium Oxysporum f. sp. ciceri Causing Wilt in Chickpea
260			_aDahrwad _bUniversity of Agricultural Science _c2024
300			_a123 _c32 Cms
520			_aABSTRACT Fusarium wilt is one of the most consequential diseases affecting chickpea yield in India, which is caused by Fusarium oxysporum f. sp. ciceri (Foc). Endophytes are among the promising plant growth-promoting and biocontrol potential microbes. Bacterial endophytes can be defined as those bacteria that colonize the internal tissue of the plant showing no external sign of infection or negative effect on their host. In the present investigation, screening of advanced breeding lines against chickpea wilt, isolation and characterization of bacterial endophytes, in vitro evaluation of endophytes against Foc, effect of seed treatment with bacterial endophytes for the management of wilt disease in sick pots and growth promotion by bacterial endophytes were studied. Screening of 70 advanced breeding lines against chickpea wilt resulted in 13 resistant lines. A total of 16 bacterial endophytic isolates were isolated from chickpea. Morphological characterization revealed that the colony colour of isolates varied from light white to creamy white. Biochemical tests revealed that, all the isolates were gram positive and showed positive result for H2S production, gelatin liquefaction, catalase test, oxidase test and negative result for indole production test. Based on molecular characterization, one strain each of Bacillus mojavensis, B. zhangzhouensis, B. velezensis, B. pumilus, B. safensis, B. licheniformis, Lysinibacilllus fusiformis, two strains of B. tequilensis, three strains of B. cereus and four strains of B. subtilis were identified. In vitro evaluation of bacterial endophytes against Foc revealed that, maximum growth inhibition of Foc was by B. subtilis CBE15 (70.33%) followed by B. subtilis CBE14 (65.57%) and B. safensis CBE11 (60.95%), respectively. Seed treatment with bacterial endophytes B. subtilis CBE15 + B. subtilis CBE14 + B. tequilensis CBE11 (10 g/kg of seeds) recorded minimum wilt incidence of 6.67 per cent. Growth promotional potential of these bacterial endophytes was also established in pot culture experiments.
700			_aKumbar Basamma
942			_cTH _h632.3 _kNIS _n0 _6632_300000000000000
999			_c70690 _d70690