000 02540nam a2200217 4500
003 OSt
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040 _cUAS Dharwad
041 _aEnglish
082 _a634.9
_bNIK
100 _aNikhil N.
245 _aClonal Propagation Through Leaves in Selected Medicinal Plant Species
250 _aM.Sc. (Forest)
260 _aDharwad
_bUniversity of Agricultural Sciences
_c2024
300 _a109
_c32 Cms
520 _aABSTRACT Clonal forestry is a powerful tool for enhancing forest productivity, sustainability, and economic value while also contributing to conservation and research efforts. It ensures that the propagated plants are genetically identical to the parent tree. This study was conducted during the post-rainy and summer seasons from September 2023 to May 2024. Mature, disease-free leaves of six species, namely Celastrus paniculatus, Premna integrifolia, Morinda citrifolia, Bridelia stipularis, Swietenia mahagoni, and Cinnamon camphora, were treated with different concentrations of hormones, and leaf cuttings were propagated in coir pith using closed media sachet technique. Various combinations of growth hormones IBA (250 ppm and 500 ppm) and 6-BAP (5 ppm, 10 ppm, and 15 ppm) were applied to the leaf petiole and surface, respectively. Observations after 120 days such as percentage of leaves survived, rooted, and sprouted; number of roots and sprouts per leaf; and the length of the longest root and sprout and biomass estimation. Though rooting was achieved in all species in either season, sprouting and complete plant regeneration were possible in three species: Morinda citrifolia, Bridelia stipularis, and Cinnamon camphora. Perhaps for the first time a complete regeneration has been achieved in these species. For most of the species IBA 250 ppm was found to be effective in inducing root and control condition is best suited for sprouting of leaves. The best season for rooting and sprouting is summer except for swietenia mahagoni. Clonal propagation offers advantages like genetic potential capture and yield improvement but faces limitations such as reduced biodiversity and susceptibility to pests. Leaf propagation emerges as an efficient method, ideal for rapid shooting and raising endangered species, with implications for improved plant multiplication and genetic preservation.
650 _aDepartment of Forest Biology
700 _aVasudeva R.
942 _cTH
_eM.Sc. (Forest)
_h634.9
_kNIK
_n0
_6634_900000000000000
999 _c70713
_d70713